The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Belongs to the nesprin family. Contains 1 KASH domain. Contains 2 spectrin repeats.
The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.