Overview

  • Product name

    Anti-Nesprin3 antibody [EPR15623-5]
    See all Nesprin3 primary antibodies
  • Description

    Rabbit monoclonal [EPR15623-5] to Nesprin3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nesprin3 aa 750-950. The exact sequence is proprietary.
    Database link: Q6ZMZ3

  • Positive control

    • Jurkat, 293 and HeLa cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15623-5
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).

Target

  • Function

    Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
  • Sequence similarities

    Belongs to the nesprin family.
    Contains 1 KASH domain.
    Contains 2 spectrin repeats.
  • Domain

    The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • Cellular localization

    Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf49 antibody
    • Chromosome 14 open reading frame 49 antibody
    • FLJ25605 antibody
    • MGC75267 antibody
    • Nesp3 antibody
    • Nesprin 3 antibody
    • Nesprin-3 antibody
    • NET53 antibody
    • Nuclear Envelope Spectrin Repeat Protein 3 antibody
    • Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody
    • SYNE3 antibody
    • SYNE3_HUMAN antibody
    see all

Images

  • All lanes : Anti-Nesprin3 antibody [EPR15623-5] (ab186751) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 112 kDa

References

ab186751 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186751.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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