Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15623-5] to Nesprin3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Nesprin3 antibody [EPR15623-5] - BSA and Azide free
See all Nesprin3 primary antibodies
DescriptionRabbit monoclonal [EPR15623-5] to Nesprin3 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Nesprin3 aa 750-950. The exact sequence is proprietary.
Database link: Q6ZMZ3
Ab250902 is the carrier-free version of ab186751. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250902 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab250902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).|
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Sequence similaritiesBelongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats.
DomainThe KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Cellular localizationNucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
- Information by UniProt
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
All lanes : Anti-Nesprin3 antibody [EPR15623-5] (ab186751) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 112 kDa
This data was developed using ab186751, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250902 has not yet been referenced specifically in any publications.