Product nameAnti-Nesprin3 antibody [EPR15623]
See all Nesprin3 primary antibodies
DescriptionRabbit monoclonal [EPR15623] to Nesprin3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Nesprin3 aa 750-950. The exact sequence is proprietary.
Database link: Q6ZMZ3
- Jurkat and 293 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 112 kDa.|
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Sequence similaritiesBelongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats.
DomainThe KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Cellular localizationNucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
- Information by UniProt
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
All lanes : Anti-Nesprin3 antibody [EPR15623] (ab186746) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 112 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
ab186746 has not yet been referenced specifically in any publications.