Product nameAnti-Nesprin3 antibody [Nsp3]
See all Nesprin3 primary antibodies
DescriptionMouse monoclonal [Nsp3] to Nesprin3
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse
Does not react with: Human, Zebrafish
Fusion protein encoding the seventh spectrin repeat of Nesprin3 and a proprietary protein.
- Mouse embryonic fibroblasts.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab123031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. FITC-conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) may be used as detection reagents.|
|IHC-P||1/100 - 1/1000.|
|IHC-Fr||1/100 - 1/1000. fixed with methanol|
|ICC/IF||Use at an assay dependent concentration.|
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Sequence similaritiesBelongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats.
DomainThe KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Cellular localizationNucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
- Information by UniProt
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
This product has been referenced in:
- Pieuchot L et al. Curvotaxis directs cell migration through cell-scale curvature landscapes. Nat Commun 9:3995 (2018). Read more (PubMed: 30266986) »
- Alam SG et al. The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity. Sci Rep 6:38063 (2016). ICC/IF . Read more (PubMed: 27905489) »