Overview

  • Product name

    Anti-Nesprin3 antibody [Nsp3]
    See all Nesprin3 primary antibodies
  • Description

    Mouse monoclonal [Nsp3] to Nesprin3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human, Zebrafish
  • Immunogen

    Fusion protein encoding the seventh spectrin repeat of Nesprin3 and a proprietary protein.

  • Positive control

    • Mouse embryonic fibroblasts.

Properties

Applications

Our Abpromise guarantee covers the use of ab123031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. FITC-conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) may be used as detection reagents.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000. fixed with methanol
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
  • Sequence similarities

    Belongs to the nesprin family.
    Contains 1 KASH domain.
    Contains 2 spectrin repeats.
  • Domain

    The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • Cellular localization

    Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf49 antibody
    • Chromosome 14 open reading frame 49 antibody
    • FLJ25605 antibody
    • MGC75267 antibody
    • Nesp3 antibody
    • Nesprin 3 antibody
    • Nesprin-3 antibody
    • NET53 antibody
    • Nuclear Envelope Spectrin Repeat Protein 3 antibody
    • Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody
    • SYNE3 antibody
    • SYNE3_HUMAN antibody
    see all

Images

  • ab123031 staining Nesprin3 in cultured mouse embryonic fibroblast by Immunofluorescence (green). The nuclear DNA is counterstained with DAPI (blue).

References

This product has been referenced in:

See all 3 Publications for this product

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