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WB image? samples tested? used human medulla blastoma celline (cerebellum, brain) 50 ug protein loaded Ab: 1/1000 4 degree over night (weekend) sees band at 90 kDa, but predicted MW is 177 kDa
Asked on Nov 03 2011
Thank you for your patience. I have attached the test results from the lab. We performed an immunoblotting experiment on a cell extract of U251 cells. The attached Western blot image clearly indicates a Nestin band with a MW of approximately 250 kDa. The test was a result of a 1:1000 antibody dilution prepared from a purified stock solution of 1mg/ml. The protein might be run higher than theoretically predicted due to the large number of phosphorylation sites (about 30). This however would not explain why you see a band at only 90 kDa. Please let me know if you have tried anything else in the meantime and receivd different results. If not and the antibody was ordered within the last 6 month, we can discuss a replacement, credit or refund. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 03 2011