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Further to my email, I am now interested in a couple more antibodies. Please could you give me the best price for the following items:
c-Myc (Y69) Ref: ab32072
Nestin (10C2) Ref: ab22035
HIF1 alpha (H1alpha67) Ref: ab80544
Please also include volumes of each and recommended dilutions for IHC-P.
Asked on Dec 13 2012
Thank you for contacting us.
1. Anti-Nestin antibody [10C2] (ab22035) and Anti-HIF1 alpha antibody [H1alpha67] - BSA and Azide free (ab80544):
Volume: 100 µg at 1 mg/ml
We recommend to start with a concentration of 5 ug/ml. This will need further optimisation depending on samples and protocol.
2. Anti-c-Myc antibody [Y69] (ab32072)
Volume: 100 ul with a concentration from 0.1120 mg/ml for lot GR85451-5 (please refer to this lot when ordering).
We recommend to use this antibody in a dilution of 1/50 in IHC-P. As said previously this recommendation will need optimisation for every experiment by user.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447
Answered on Dec 13 2012