Anti-Nestin antibody [196908] (ab6320)
Key features and details
- Mouse monoclonal [196908] to Nestin
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nestin antibody [196908]
See all Nestin primary antibodies -
Description
Mouse monoclonal [196908] to Nestin -
Host species
Mouse -
Specificity
Based on the result of immunocytochemistry on mouse and rat neural stem cells, this antibody has no reactivity with rodent Nestin. The clone number has been updated from (3k1) to (196908) both clone numbers name the same antibody clone. -
Tested Applications & Species
Application Species ICC/IF HumanWB Human -
Immunogen
Nestin-transfected NS0 cells transfected with a human Nestin fragment aa residues 618-1618.
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Positive control
- Human neural progenitors and A172 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: PBS, 5% Trehalose -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
196908 -
Myeloma
NS0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 177 kDa.
|
ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 177 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
Tissue specificity
CNS stem cells. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Database links
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
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Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
Images
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All lanes : Anti-Nestin antibody [196908] (ab6320) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NES knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 177 kDaLanes 1 - 2: Merged signal (red and green). Green - ab6320 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab6320 was shown to recognize NES in wild-type HAP1 cells as signal was lost at the expected MW in NES knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Ab6320 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemical analysis of human fetal neural progenitor cells, labeling Nestin with ab6320 (10µg/ml). Immunostaining was for 3 hours at room temperature. Counterstaining with DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (35)
ab6320 has been referenced in 35 publications.
- Zhang J et al. Biological Characterization and Therapeutics for Subscalp Recurrent in Intracranial Glioblastoma. Onco Targets Ther 13:9085-9099 (2020). PubMed: 32982297
- Jiang Y et al. Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia. EBioMedicine 48:36-48 (2019). PubMed: 31631037
- Wen N et al. Bromodomain inhibitor jq1 induces cell cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway. Int J Oncol 55:879-895 (2019). PubMed: 31485609
- Jiang Y et al. NFAT1-Mediated Regulation of NDEL1 Promotes Growth and Invasion of Glioma Stem-like Cells. Cancer Res 79:2593-2603 (2019). PubMed: 30940662
- Yuan Y et al. A six-inhibitor culture medium for improving naïve-type pluripotency of porcine pluripotent stem cells. Cell Death Discov 5:104 (2019). PubMed: 31240131