Western blot abreview for Anti-Nestin antibody [3k1] - Neural Stem Cell Marker

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary glioblastoma cell lines)
Loading amount
25 µg
Specification
Primary glioblastoma cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse IgG HRP Cell signalling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 180, 190 kDa
Positive control
Glioblastoma multiforme cell line grown as neurosphere in stem cell like media

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Submitted Aug 03 2012

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