Immunocytochemistry/ Immunofluorescence abreview for Anti-Nestin antibody [3k1] - Neural Stem Cell Marker

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (hair follicle culture)
Specification
hair follicle culture
Permeabilization
Yes - methanol
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Diluent: PBS-T+1%BSA
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/500

Additional data

Additional Notes
The image shows nestin-postive cells migrating out of the cultured hair follicle.

Mr. Heiko Locher

Verified customer

Submitted Sep 23 2013

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