Overview

  • Product name
    Anti-Nestin antibody [2C1.3A11]
    See all Nestin primary antibodies
  • Description
    Mouse monoclonal [2C1.3A11] to Nestin
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    A 150 amino acid fragment from the cloned human Nestin.

  • General notes


    This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 177 kDa.
Flow Cyt 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificity
    CNS stem cells.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Developmental stage
    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunofluorescence staining of U251 cells (glioblastoma cell line) stained using ab18102(green) and bisbenzimide (blue)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.

    • Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
      All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/1000 dilution

      Lane 1 : Total rat brain protein at 5 µg
      Lane 2 : Human CNS progenitor cell protein at 1 µg
      Lane 3 : Human CNS progenitor cell protein at 5 µg

      Predicted band size: 177 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of xenograft tumor cells labelling Nestin with ab18102.

    • Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Overlay histogram showing SH-SY5Y cells stained with ab18102 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18102, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry of Anti-Nestin on formalin fixed paraffin embedded prostate nerve tissue using ab18102

    References

    This product has been referenced in:
    • Jaraíz-Rodríguez M  et al. A Short Region of Connexin43 Reduces Human Glioma Stem Cell Migration, Invasion, and Survival through Src, PTEN, and FAK. Stem Cell Reports 9:451-463 (2017). Read more (PubMed: 28712848) »
    • Sherry MM  et al. STAT3 is required for proliferation and maintenance of multipotency in glioblastoma stem cells. Stem Cells 27:2383-92 (2009). WB ; Human . Read more (PubMed: 19658181) »

    See all 3 Publications for this product

    Publishing research using ab18102? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

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    Votes: Highest First

    Flow Cytometry abreview for Anti-Nestin antibody [2C1.3A11] - Neural Stem Cell Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (Neural Progenitor cells (from human ES cells))
    Specification
    Neural Progenitor cells (from human ES cells)
    Preparation
    Cell harvesting/tissue preparation method: Cell Dissociation Buffer
    Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 70% Methanol (ice cold)
    Gating Strategy
    FSC vs SSC on negative control sample
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    Abcam user community

    Verified customer

    Submitted Jan 03 2012

    Yes, that would be for the nestin antibodies only. (ab6320 & ab18102) If you can find any specific FACS protocol for one of these, then it may be decisive in my choice. Up to know, I do not have any information for choosing between them. Thus I am...

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    I am please to be able to share the following protocol for ab18102for Flow cytometry: Fix cells with 4% Paraformaldehyde for 30 min at room temperature (RT) Wash twice with 1% PBS at 1500rpm/5min/RT Permeabilize cells with 0.02% Triton X-100 in 1%P...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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