- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-Nestin antibody [2C1.3A11]
See all Nestin primary antibodies -
DescriptionMouse monoclonal [2C1.3A11] to Nestin
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-P, IPmore details
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Species reactivityReacts with: Human
Does not react with: Rat -
Immunogen
A 150 amino acid fragment from the cloned human Nestin.
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General notes
This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesThis highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
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ClonalityMonoclonal
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Clone number2C1.3A11
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab18102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/500. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 177 kDa. | |
| Flow Cyt | 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
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Tissue specificityCNS stem cells.
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Sequence similaritiesBelongs to the intermediate filament family.
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Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
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Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Database links
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
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Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)](https://www.abcam.com/ps/products/18/ab18102/Images/Nestin-Primary-antibodies-ab18102-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)Immunofluorescence staining of U251 cells (glioblastoma cell line) stained using ab18102(green) and bisbenzimide (blue) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)](https://www.abcam.com/ps/products/18/ab18102/Images/ab18102-219273-ab18102ihcp2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.
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![Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)](https://www.abcam.com/ps/products/18/ab18102/Images/ab18102-219266-ab18102wb.jpg)
Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/1000 dilution
Lane 1 : Total rat brain protein at 5 µg
Lane 2 : Human CNS progenitor cell protein at 1 µg
Lane 3 : Human CNS progenitor cell protein at 5 µg
Predicted band size: 177 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)](https://www.abcam.com/ps/products/18/ab18102/Images/ab18102-219267-ab18102ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of xenograft tumor cells labelling Nestin with ab18102.
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![Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)](https://www.abcam.com/ps/products/18/ab18102/Images/Nestin-Primary-antibodies-ab18102-1.jpg)
Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)Overlay histogram showing SH-SY5Y cells stained with ab18102 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18102, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)](https://www.abcam.com/ps/products/18/ab18102/Images/Nestin-Primary-antibodies-ab18102-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)Immunohistochemistry of Anti-Nestin on formalin fixed paraffin embedded prostate nerve tissue using ab18102
Protocols
References
This product has been referenced in:
- Jaraíz-Rodríguez M et al. A Short Region of Connexin43 Reduces Human Glioma Stem Cell Migration, Invasion, and Survival through Src, PTEN, and FAK. Stem Cell Reports 9:451-463 (2017). Read more (PubMed: 28712848) »
- Sherry MM et al. STAT3 is required for proliferation and maintenance of multipotency in glioblastoma stem cells. Stem Cells 27:2383-92 (2009). WB ; Human . Read more (PubMed: 19658181) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"