Overview

  • Product name

    Anti-Nestin antibody [7A3]
    See all Nestin primary antibodies
  • Description

    Rat monoclonal [7A3] to Nestin
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Mouse E16 embryonic cerebral cortex extracts

  • Positive control

    • Neural progenitor cells from mouse fetal brain.
  • General notes


    ab81462 is very useful for immunostaining of mouse embryonic brain. It has very low background in immunostaining using mouse tissues and is also useful for doubleb staining with mouse and rabbit antibodies.

Properties

Applications

Our Abpromise guarantee covers the use of ab81462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificity

      CNS stem cells.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Developmental stage

      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications

      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links

    • Alternative names

      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Images

    • Primary culture of neural progenitor cells from mouse fetal brain stained with ab81462.
    • Primary culture of neural progenitor cells from mouse fetal brain, without staining (bright field image).

    References

    This product has been referenced in:

    • Ngwenya LB  et al. Implantation of Neuronal Stem Cells Enhances Object Recognition without Increasing Neurogenesis after Lateral Fluid Percussion Injury in Mice. Stem Cells Int 2018:4209821 (2018). ICC/IF ; Mouse . Read more (PubMed: 29531536) »
    • Betlazar C  et al. Cellular Sources and Regional Variations in the Expression of the Neuroinflammatory Marker Translocator Protein (TSPO) in the Normal Brain. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30208620) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Bone marrow)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    Yes - Triton, 5min
    Specification
    Bone marrow
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 03 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cor1 cells)
    Specification
    Cor1 cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% TWEEN
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted May 25 2011

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain and kidney)
    Specification
    Brain and kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 13 2010

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