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    nestin-antibody-ab134017.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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Anti-Nestin antibody (ab134017)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (17)

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Immunocytochemistry - Anti-Nestin antibody (ab134017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)
  • Western blot - Anti-Nestin antibody (ab134017)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)

Key features and details

  • Chicken polyclonal to Nestin
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgY

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Overview

  • Product name

    Anti-Nestin antibody
    See all Nestin primary antibodies
  • Description

    Chicken polyclonal to Nestin
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Three synthetic peptides, conjugated to KLH, corresponding to regions within Mouse Nestin (NP_057910).

  • Positive control

    • e13 mouse brain
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    IgY fractions were purified from the yolks and IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 300 µg/ml, and the preparation filter-sterilized.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Compatible Secondaries

    • Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
    • Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    • Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)

Applications

Our Abpromise guarantee covers the use of ab134017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10000 - 1/20000.
ICC 1/10000 - 1/20000.
WB 1/20000 - 1/50000. Predicted molecular weight: 177 kDa.

Target

  • Function

    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificity

    CNS stem cells.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10763 Human
    • Entrez Gene: 18008 Mouse
    • Omim: 600915 Human
    • SwissProt: P48681 Human
    • SwissProt: Q6P5H2 Mouse
    • SwissProt: P21263 Rat
    • Unigene: 527971 Human
    • Unigene: 331129 Mouse
    • Unigene: 9701 Rat
    see all
  • Alternative names

    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Images

  • Immunocytochemistry - Anti-Nestin antibody (ab134017)
    Immunocytochemistry - Anti-Nestin antibody (ab134017)

    Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)

    Immunohistochemical analysis of a tissue section through an e13 mouse brain labelling Nestin (green staining) in neural stem cells of the ventricular zone of the CNS with ab134017 at 1/10000 dilution. DAPI (blue staining) allows visualization of nuclei.

  • Western blot - Anti-Nestin antibody (ab134017)
    Western blot - Anti-Nestin antibody (ab134017)
    Anti-Nestin antibody (ab134017) at 1/10000 dilution + neonatal mouse brain

    Predicted band size: 177 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (17)

    Publishing research using ab134017? Please let us know so that we can cite the reference in this datasheet.

    ab134017 has been referenced in 17 publications.

    • Wang S  et al. Integrin avß5 Internalizes Zika Virus during Neural Stem Cells Infection and Provides a Promising Target for Antiviral Therapy. Cell Rep 30:969-983.e4 (2020). PubMed: 31956073
    • Su J  et al. Cellular senescence mediates the detrimental effect of prenatal dexamethasone exposure on postnatal long bone growth in mouse offspring. Stem Cell Res Ther 11:270 (2020). PubMed: 32631432
    • Philip DL  et al. RGD-Functionalized Nanofibers Increase Early GFAP Expression during Neural Differentiation of Mouse Embryonic Stem Cells. Biomacromolecules 20:1443-1454 (2019). PubMed: 30726667
    • Boos JA  et al. Microfluidic Multitissue Platform for Advanced Embryotoxicity Testing In Vitro. Adv Sci (Weinh) 6:1900294 (2019). PubMed: 31380185
    • Pierozan P & Karlsson O Mitotically heritable effects of BMAA on striatal neural stem cell proliferation and differentiation. Cell Death Dis 10:478 (2019). PubMed: 31209203
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Nestin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Rat bearing rat brain tumor 9L)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 0.2%
    Specification
    Rat bearing rat brain tumor 9L
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted May 04 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Nestin antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.5 % TritonX100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruth Beckervordersandforth

    Verified customer

    Submitted Aug 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nestin antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Salivary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA Ph9 - Also tried Sodium Citrate Ph6
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Salivary gland
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Dr. Isabelle Miletich

    Verified customer

    Submitted Jul 06 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Nestin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Human Tissue sections (brain mouse bearing human tumor)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 0.2%
    Specification
    brain mouse bearing human tumor
    Blocking step
    BSA1% + DK serum 5% as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Jan 11 2016

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