Anti-Nestin antibody (ab134017)
- Datasheet
- References (13)
- Protocols
Overview
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Product name
Anti-Nestin antibody
See all Nestin primary antibodies -
Description
Chicken polyclonal to Nestin -
Host species
Chicken -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Three synthetic peptides, conjugated to KLH, corresponding to regions within Mouse Nestin (NP_057910).
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Positive control
- e13 mouse brain
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
IgY fractions were purified from the yolks and IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 300 µg/ml, and the preparation filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/10000 - 1/20000. | |
| ICC | 1/10000 - 1/20000. | |
| WB | 1/20000 - 1/50000. Predicted molecular weight: 177 kDa. |
Target
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Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
Tissue specificity
CNS stem cells. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Database links
- Entrez Gene: 10763 Human
- Entrez Gene: 18008 Mouse
- Omim: 600915 Human
- SwissProt: P48681 Human
- SwissProt: Q6P5H2 Mouse
- SwissProt: P21263 Rat
- Unigene: 527971 Human
- Unigene: 331129 Mouse
see all -
Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
Images
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Immunocytochemistry - Anti-Nestin antibody (ab134017)Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)Immunohistochemical analysis of a tissue section through an e13 mouse brain labelling Nestin (green staining) in neural stem cells of the ventricular zone of the CNS with ab134017 at 1/10000 dilution. DAPI (blue staining) allows visualization of nuclei.
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Western blot - Anti-Nestin antibody (ab134017)Anti-Nestin antibody (ab134017) at 1/10000 dilution + neonatal mouse brain
Predicted band size: 177 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.
Protocols
References
This product has been referenced in:
- Philip DL et al. RGD-Functionalized Nanofibers Increase Early GFAP Expression during Neural Differentiation of Mouse Embryonic Stem Cells. Biomacromolecules 20:1443-1454 (2019). Read more (PubMed: 30726667) »
- Boos JA et al. Microfluidic Multitissue Platform for Advanced Embryotoxicity Testing In Vitro. Adv Sci (Weinh) 6:1900294 (2019). Read more (PubMed: 31380185) »
