Overview

  • Product name

    Anti-Nestin antibody - C-terminal
    See all Nestin primary antibodies
  • Description

    Rabbit polyclonal to Nestin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide within Human Nestin aa 1600 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P48681

  • Positive control

    • IHC-P: Human liver hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificity

    CNS stem cells.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links

  • Alternative names

    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human liver hepatoma tissue stained for Nestin using ab227777 at 1/100 dilution in immunohistochemical analysis.

References

ab227777 has not yet been referenced specifically in any publications.

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