Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)

Overview

  • Product name
    Anti-Nestin antibody - Neural Stem Cell Marker
    See all Nestin primary antibodies
  • Description
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • Host species
    Rabbit
  • Specificity
    Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Hamster
  • Immunogen

    Synthetic peptide:

    ALETESQDSAEPSGSEE

    , corresponding to amino acids 1481-1497 of Human Nestin.

  • General notes
    ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab7659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificity
      CNS stem cells.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Developmental stage
      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Images

    • Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg

      Secondary
      IRDye 800
      conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 176 kDa
      Observed band size: 200 kDa (why is the actual band size different from the predicted?)

    • All lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml

      Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
      Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP at 1/20000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 176 kDa
      Observed band size: 230 kDa (why is the actual band size different from the predicted?)


      Exposure time: 10 minutes


      A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.

    References

    This product has been referenced in:
    • Veron AD  et al. Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera. BMC Vet Res 14:17 (2018). ICC/IF . Read more (PubMed: 29343270) »
    • Dorraji SE  et al. Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis. Sci Rep 8:7861 (2018). Read more (PubMed: 29777158) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting Abcam regarding ab7659. These applications have been listed as unsuitable as we have obtained a range of feedback stating that the antibody is staining nonspecifically in these applications.  If you have not used this a...

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    Ab7659 is a Fast Track antibody - Fast Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen and have been released early at a reduced price. Ab7659 has not yet been tested for applica...

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    This antibody is an investigative grade antibody, which means it has not been fully characterised by Abcam but has been released due to public interest. It has only been tested in ELISA so far and its lower price reflects that. It has only been shown t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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