Key features and details
- Rabbit polyclonal to Nestin - Neural Stem Cell Marker
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Nestin antibody - Neural Stem Cell Marker
See all Nestin primary antibodies
DescriptionRabbit polyclonal to Nestin - Neural Stem Cell Marker
SpecificityThe immunoreactivity detected with Rabbit polyclonal to Nestin (ab92391) does not overlap in with that of the mature neuronal markers such as MAP2, NeuN, Neurofilament L and Beta-3-tublin (data available on request).
Tested applicationsSuitable for: ICC/IFmore details
Unsuitable for: IHC-FoFr or IHC-P
Species reactivityReacts with: Rat
Predicted to work with: Human
Synthetic peptide corresponding to Human Nestin aa 1600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in P0 rat retinal cell culture and E16 rat hippocampal cell culture.
General notesab92391 is raised using the same immunogen as ab5968. Batches sold under ab92391 have passed QC testing in ICC/IF only. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab92391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Tissue specificityCNS stem cells.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
- Information by UniProt
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Immunocytochemical localisation of Nestin using ab92391 on P0 rat retinal culture. The image shows a nestin positive retinal ganglionic cell from P0 retina that has been grown for 7 days on a glass coverslip. Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000).
Immunocytochemical localisation of Nestin using ab92391 on E16 rat hippocampal cell culture. The image shows nestin positive rat hippocampal cells isolated from embryonic day 16 (grown in serum free conditions for 2-3 weeks). Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000). DAPI in blue stains nuclei.
ICC/IF image of ab92391 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab92391 has been referenced in 18 publications.
- Lee JY et al. Human parthenogenetic neural stem cell grafts promote multiple regenerative processes in a traumatic brain injury model. Theranostics 9:1029-1046 (2019). PubMed: 30867814
- Bott CJ et al. Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity. Mol Biol Cell 30:1214-1229 (2019). PubMed: 30840538
- Yang S et al. In Vitro Monolayer Culture of Dispersed Neural Stem Cells on the E-Cadherin-Based Substrate with Long-Term Stemness Maintenance. ACS Omega 4:18136-18146 (2019). PubMed: 31720516
- Michel N et al. Imaging Flow Cytometry Quantifies Neural Genome Dynamics. Cytometry A 95:825-835 (2019). PubMed: 31063256
- Hu YD et al. Comparison of the properties of neural stem cells of the hippocampus in the tree shrew and rat in vitro. Mol Med Rep 17:5676-5683 (2018). PubMed: 29436662