Overview

  • Product name

    Anti-Nestin antibody - Neural Stem Cell Marker
    See all Nestin primary antibodies
  • Description

    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • Host species

    Rabbit
  • Specificity

    The immunoreactivity detected with Rabbit polyclonal to Nestin (ab92391) does not overlap in with that of the mature neuronal markers such as MAP2, NeuN, Neurofilament L and Beta-3-tublin (data available on request).
  • Tested applications

    Suitable for: ICC/IFmore details
    Unsuitable for: IHC-FoFr or IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Nestin aa 1600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab19375)

  • Positive control

    • This antibody gave a positive signal in P0 rat retinal cell culture and E16 rat hippocampal cell culture.
  • General notes

    ab92391 is raised using the same immunogen as ab5968. Batches sold under ab92391 have passed QC testing in ICC/IF only. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
  • Application notes
    Is unsuitable for IHC-FoFr or IHC-P.
  • Target

    • Function

      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificity

      CNS stem cells.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Developmental stage

      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications

      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links

    • Alternative names

      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Images

    • Immunocytochemical localisation of Nestin using ab92391 on P0 rat retinal culture. The image shows a nestin positive retinal ganglionic cell from P0 retina that has been grown for 7 days on a glass coverslip. Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000).
    • Immunocytochemical localisation of Nestin using ab92391 on E16 rat hippocampal cell culture. The image shows nestin positive rat hippocampal cells isolated from embryonic day 16 (grown in serum free conditions for 2-3 weeks). Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000). DAPI in blue stains nuclei.
    • ICC/IF image of ab92391 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:

    • Lee JY  et al. Human parthenogenetic neural stem cell grafts promote multiple regenerative processes in a traumatic brain injury model. Theranostics 9:1029-1046 (2019). Read more (PubMed: 30867814) »
    • Tian H  et al. Yi-nao-jie-yu Prescription Exerts a Positive Effect on Neurogenesis by Regulating Notch Signals in the Hippocampus of Post-stroke Depression Rats. Front Psychiatry 9:483 (2018). Read more (PubMed: 30386260) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney, glomerulus)
    Permeabilization
    No
    Specification
    Kidney, glomerulus
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 27 2019

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (neurosphere)
    Permeabilization
    Yes - 0.025% TritonX
    Specification
    neurosphere
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted May 04 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (corneal endothelium)
    Specification
    corneal endothelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 1% triton
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C

    Dr. Zhiguo He

    Verified customer

    Submitted Nov 01 2012

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No

    Dr. Sophie Pezet

    Verified customer

    Submitted Sep 21 2012

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None

    Dr. Sophie Pezet

    Verified customer

    Submitted May 26 2010

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