Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - Triton-X 100
Specification
Brain
Blocking step
Donkey serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Donkey serum
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: AffiniPure Donkey Anti-Mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
No signal whatsoever, despite multiple attempts with a protocol that has worked well with other Abcam antibodies in the past.
Abcam response
ab6142 is tested with rat samples and guaranteed to work. We recommend fixing the sample with ice cold acetone or methanol or mixture of both at 1:1 ratio. The end user of this review has been contacted with suggestions, and a free product has been offered in line with our Abpromise guarantee. In case of any question about troubleshooting contact Abcam Scientific Support team.
Alyssa Cortella
Verified customer
Submitted Aug 24 2018