Anti-Nestin antibody [SP103] (ab105389)
- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-Nestin antibody [SP103]
See all Nestin primary antibodies -
DescriptionRabbit monoclonal [SP103] to Nestin
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Dog
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Immunogen
Synthetic peptide within Human Nestin aa 1600 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P48681 -
Positive control
- IHC-P: Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. WB: HeLa cell lysate. Flow Cyt: SH-SY5Y cells. ICC/IF: SKNSH cells.
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General notes
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 0.0268% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP103
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab105389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200. | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/100. Predicted molecular weight: 177 kDa. | |
| IHC-P | 1/100. Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
Target
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FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
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Tissue specificityCNS stem cells.
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Sequence similaritiesBelongs to the intermediate filament family.
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Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
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Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Database links
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
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Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-249536-105389IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human kidney tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/Nestin-Primary-antibodies-ab105389-1.jpg)
Western blot - Anti-Nestin antibody [SP103] (ab105389)Anti-Nestin antibody [SP103] (ab105389) at 1/100 dilution + HeLa cell lysate
Predicted band size: 177 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308747-3895.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human endometrium tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/Nestin-Primary-antibodies-ab105389-23.jpg)
Flow Cytometry - Anti-Nestin antibody [SP103] (ab105389)Overlay histogram showing SH-SY5Y cells stained with ab105389 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105389, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/Nestin-Primary-antibodies-ab105389-22.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] (ab105389)ICC/IF image of ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308746-3894.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human colon tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308750-3898.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human prostate tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308749-3897.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human lung tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308744-3893.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human breast tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308751-3899.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human skin tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308748-3896.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308743-3892.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human cervix tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/ab105389-308742-3891.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)Formalin-fixed, paraffin-embedded human ovary tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)](https://www.abcam.com/ps/products/105/ab105389/Images/Nestin-Primary-antibodies-ab105389-11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)This image is courtesy of Hongwei Shao by Abreview.ab105389 staining Nestin in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked, then incubated with ab105389 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-rabbit polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.
Protocols
References
This product has been referenced in:
- Mesci P et al. Blocking Zika virus vertical transmission. Sci Rep 8:1218 (2018). Read more (PubMed: 29352135) »
- Colini Baldeschi A et al. Atrial Natriuretic Peptide Acts as a Neuroprotective Agent in in Vitro Models of Parkinson's Disease via Up-regulation of the Wnt/ß-Catenin Pathway. Front Aging Neurosci 10:20 (2018). WB, ICC/IF . Read more (PubMed: 29449807) »
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