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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Yes - 0.25% Triton X-100
DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Non-Abcam antibody was used: Alexa Flour594 Donkey anti-rabbit IgG
Host species: Donkey
Conjugation: Alexa Fluor® 594
Red: Nestin positive cells.
Blue: DAPI stained nucleus.
Submitted Nov 09 2011