Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nestin antibody [SP103]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min, 100C
Permeabilization
No

Other product details

Dilution
1/200
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse, anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Procedure automated on Leica Microsystems Bond III IHC system, 20 minute antibody incubation, using Refine Polymer detection. No permeabilization or blocking necessary, other than endogenous peroxidase with H2O2.
Numerous antigen retrieval regimes tested - EDTA gave best signal/background

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Submitted Nov 22 2011

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