Immunocytochemistry/ Immunofluorescence abreview for Anti-Nestin antibody [SP103]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
1% serum, 0.1% triton, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample
Human Cell (neural progenitor cell derived from human iPSCs)
Specification
neural progenitor cell derived from human iPSCs
Permeabilization
Yes - (see blocking buffer)
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: in blocking buffer
Dilution
1/200

Secondary antibody

Dilution
1/500

Additional data

Additional Notes
iPS derived neural progenitor cells were fixed in 4% formaldehyde (10 min) and then incubated in 1% serum, 0.1% triton, 0.1% BSA in PBS for 30 mins to permeabilise the cells and block non-specific binding. The cells were then incubated with antibody (ab105389, 1/200 dilution) for 1h at room temp. The secondary antibody (red) was Donkey Anti-Rabbit IgG H&L Alexa 555 (ab150074) at 1:500 dilution and incubated for 1h at room temp. Total cells were stained with DAPI (blue). Excellent positive nestin staining was observed (red).

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Submitted Nov 22 2013

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