iPS derived neural progenitor cells were fixed in 4% formaldehyde (10 min) and then incubated in 1% serum, 0.1% triton, 0.1% BSA in PBS for 30 mins to permeabilise the cells and block non-specific binding. The cells were then incubated with antibody (ab105389, 1/200 dilution) for 1h at room temp. The secondary antibody (red) was Donkey Anti-Rabbit IgG H&L Alexa 555 (ab150074) at 1:500 dilution and incubated for 1h at room temp. Total cells were stained with DAPI (blue). Excellent positive nestin staining was observed (red).
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Submitted Nov 22 2013
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