Undifferentiated human iPS cells were lysed in RIPA buffer with protease inhibitors and performed under reducing conditions (10% gel). Following block overnight the samples were probed using an anti Nestin antibody (ab105389) at 1ug/ml, followed by incubation with the relevant secondary antibody at 1:2000. Samples were visualised using ECL for 5 mins and a single band at approx 178kDa was observed. Although undifferentiated cells should not express Nestin, there are obviously some cells within the sample that have begun differentiation.
Abcam user community
Submitted Dec 18 2013
Get resources and offers direct to your inboxSign up