I tried a number of antigen retrieval conditions (proteinase K and HIER with citrate buffer) and dilutions of antibody (1:50 and 1:100). I used DAB and FITC labeled streptavidin conjugate to visualize staining and I didn't get any signal. I don't think this antibody works in canine tissue. I noted the rating as inconclusive since I don't think the antibody was in anyway bellow average, I think is doesn't bind canine Nestin.
We are sorry that this antibody did not work for this customer.
The antibody has not been previously tested in Dog and customer received an AbTrial discount code to be used in the next Abcam order. Please do not hesitate to contact our Scientific Support team if you have any questions.
Ms. Erica Sloma
Submitted Aug 21 2014
Get resources and offers direct to your inboxSign up