Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nestin antibody [SP103]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako protein block as blocking agent for 10 minute(s) · Concentration: .25% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Dog Tissue sections (Fetal tissue)
Specification
Fetal tissue
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dako antibody diluant
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Biotinylated Goat Anti-Rabbit IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
I tried a number of antigen retrieval conditions (proteinase K and HIER with citrate buffer) and dilutions of antibody (1:50 and 1:100). I used DAB and FITC labeled streptavidin conjugate to visualize staining and I didn't get any signal. I don't think this antibody works in canine tissue. I noted the rating as inconclusive since I don't think the antibody was in anyway bellow average, I think is doesn't bind canine Nestin.
Abcam response
We are sorry that this antibody did not work for this customer. The antibody has not been previously tested in Dog and customer received an AbTrial discount code to be used in the next Abcam order. Please do not hesitate to contact our Scientific Support team if you have any questions.

Ms. Erica Sloma

Verified customer

Submitted Aug 21 2014

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