Overview

  • Product name

  • Description

    Goat polyclonal to NET1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide: DKALSGGKRKETLV, corresponding to C terminal amino acids 529-542 of Human NET1.

  • General notes

    GenBank Accession Number – NP_005854

Properties

Applications

Our Abpromise guarantee covers the use of ab5914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Can be blocked with Human NET1 peptide (ab23120).
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHG8_HUMAN antibody
    • ARHGEF8 antibody
    • GLYT2 antibody
    • Guanine nucleotide regulatory protein oncogene antibody
    • MGC114559 antibody
    • MGC127767 antibody
    • MGC53602 antibody
    • mNET1 antibody
    • Net1 antibody
    • NET1A antibody
    • Neuroepithelial cell transforming gene 1 antibody
    • Neuroepithelial cell-transforming gene 1 protein antibody
    • p65 Net1 proto oncogene antibody
    • Proto-oncogene p65 Net1 antibody
    • Rho guanine nucleotide exchange factor 8 antibody
    • Rho guanine nucleotide exchange factor GEF 8 antibody
    see all

Images

  • ab5914 (2.5 µg/ml) staining NET1 in Human Tonsil by IHC-P.  Steamed antigen retrieval with citrate buffer pH 6, AP-staining.  This image shows staining of fibrous structures.

References

This product has been referenced in:

  • Sun X  et al. Inhibition of NET-1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Exp Ther Med 17:2334-2340 (2019). Read more (PubMed: 30867719) »
  • Levi L  et al. Bacterial genotoxins promote inside-out integrin ß1 activation, formation of focal adhesion complexes and cell spreading. PLoS One 10:e0124119 (2015). WB . Read more (PubMed: 25874996) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up