Recombinant
RabMAb

Recombinant Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

Overview

  • Product name

    Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free
    See all Netrin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5428] to Netrin 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Netrin 1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: O95631
    (Peptide available as ab192272)

  • General notes

    Ab201324 is the carrier-free version of ab126729. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab201324 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).Can be blocked with Netrin 1 peptide (ab192272).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
    • Tissue specificity

      Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas.
    • Sequence similarities

      Contains 3 laminin EGF-like domains.
      Contains 1 laminin N-terminal domain.
      Contains 1 NTR domain.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • epididymis tissue protein Li 131P antibody
      • NET1_HUMAN antibody
      • Netrin 1, mouse, homolog of antibody
      • Netrin 1-like antibody
      • Netrin-1 antibody
      • Netrin1 antibody
      • Ntn 1 antibody
      • NTN 1L antibody
      • NTN1 antibody
      • NTN1L antibody
      • Unc6 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling Netrin 1 with purified ab126729 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126729).

    References

    ab201324 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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