Key features and details
- Rabbit polyclonal to Netrin-2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Netrin-2 antibody
DescriptionRabbit polyclonal to Netrin-2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Human plasma (IgG/HSA depleted)
Previously labelled as NTN3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa.|
FunctionNetrins control guidance of CNS commissural axons and peripheral motor axons.
Tissue specificitySpinal cord.
Sequence similaritiesContains 3 laminin EGF-like domains.
Contains 1 laminin N-terminal domain.
Contains 1 NTR domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- NET3_HUMAN antibody
- Netrin-2-like protein antibody
- Netrin-3 antibody
Immunohistochemical analysis of paraffin embedded Human oral mucosa tissue labeling Netrin-2 with ab185200 at 1/50 and counterstained with hematoxylin.
All lanes : Anti-Netrin-2 antibody (ab185200) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251MG cell line lysate
Lane 3 : Human plasma (IgG/HSA depleted)
All lanes : Swine anti-rabbit HRP conjugated antibody at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 1 minute
ab185200 has been referenced in 1 publication.
- Dominici C et al. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance. Nature 545:350-354 (2017). PubMed: 28445456