Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample
Mouse Tissue sections (Embryonic day 15 brain)
Specification
Embryonic day 15 brain
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 1% serum, 0.1% Triton X-100
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 Donkey Anti-Rabbit IgG (H+L) Anti
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/700
Abcam user community
Verified customer
Submitted May 16 2014