Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% FBS in 0.1M Tris buffer pH7.6
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes A11037 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
Image shows NeuN in red, and nuclei (DAPI) in blue. Staining was primarily nuclear with some minor cytoplasmic fluorescence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Adam Walker
Verified customer
Submitted Jun 19 2014