Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% FBS in 0.1M Tris buffer pH7.6
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes A11037 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
Image shows NeuN in red, and nuclei (DAPI) in blue. Staining was primarily nuclear with some minor cytoplasmic fluorescence.
Dr. Adam Walker
Verified customer
Submitted Jun 19 2014