Immunohistochemistry (Frozen sections) abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (brain)
Specification
brain
Permeabilization
Yes - Triton X
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA blocking Solution
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexafluor conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Very strong staining in the brain at 4dpf. Sodium Citrate antigen retrieval was used.

Dr. Ryan Macdonald

Verified customer

Submitted Aug 14 2014

Sign up