Western blot abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human neurons differentiated from iPSCs)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Human neurons differentiated from iPSCs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/Tween 0.05%
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 46-48 kDa Non-specific: 38 kDa

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Mar 01 2019

Sign up