Product nameAnti-NeuN antibody [EPR12763] - Neuronal Marker (FITC)
See all NeuN primary antibodies
DescriptionRabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: A6NFN3
- Flow Cyt: PC12 cells. ICC/IF: PC12 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 488) (ab190195)
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 647) (ab190565)
- Anti-NeuN antibody [EPR12763] (Biotin) (ab204681)
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 594) (ab207279)
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 555) (ab207281)
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 568) (ab207282)
- Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
Our Abpromise guarantee covers the use of ab223994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)
FunctionRNA-binding protein that regulates alternative splicing events.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223994 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Overlay histogram showing PC12 cells stained with ab223994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223994, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
ab223994 has not yet been referenced specifically in any publications.