Recombinant Anti-NeuN antibody [EPR21906] - Neuronal Marker (ab236870)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21906] to NeuN - Neuronal Marker
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NeuN antibody [EPR21906] - Neuronal Marker
See all NeuN primary antibodies -
Description
Rabbit monoclonal [EPR21906] to NeuN - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, hippocampus and cerebellum lysates; Mouse and rat brain, cerebral cortex, cerebellum and hippocampus lysates. IHC-P: Human cerebrum and cerebellum tissues; Mouse and rat cerebrum tissues. IHC-Fr: Mouse and rat cerebellum tissues. IP: Human cerebellum lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21906 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 34 kDa).
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IHC-P | (2) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
1/30. |
Target
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Function
RNA-binding protein that regulates alternative splicing events. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- SwissProt: A6NFN3 Human
- SwissProt: Q8BIF2 Mouse
- Unigene: 135229 Human
- Unigene: 341103 Mouse
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Alternative names
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-NeuN antibody [EPR21906] - Neuronal Marker (ab236870) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human hippocampus lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse cerebral cortex lysate
Lane 6 : Mouse cerebellum lysate
Lane 7 : Mouse hippocampus lysate
Lane 8 : Rat brain lysate
Lane 9 : Rat cerebral cortex lysate
Lane 10 : Rat cerebellum lysate
Lane 11 : Rat hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 46,48 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-2: 3 minutes; Lane 3: 92 seconds; Lanes 4-7: 26 seconds; Lanes 8-11: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:1483388; PMID:26085943).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling NeuN with ab236870 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and nuclear staining in rat cerebellum (PMID:19713214).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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NeuN was immunoprecipitated from 0.35 mg human cerebellum lysate with ab236870 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236870 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human cerebellum lysate 10 µg (Input).
Lane 2: ab236870 IP in human cerebellum lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236870 in human cerebellum lysate (-).Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:1483388; PMID:26085943).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling NeuN with ab236870 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and nuclear staining in mouse cerebellum (PMID:19713214).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of rat cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of mouse cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of human cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab236870 has been referenced in 3 publications.
- Qi Y et al. Uncovering the Potential Mechanisms of Coptis chinensis Franch. for Serious Mental Illness by Network Pharmacology and Pharmacology-Based Analysis. Drug Des Devel Ther 16:325-342 (2022). PubMed: 35173416
- Lu Q et al. Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticity. iScience 25:104201 (2022). PubMed: 35479414
- Yi M et al. Downregulated lncRNA CRNDE contributes to the enhancement of nerve repair after traumatic brain injury in rats. Cell Cycle 18:2332-2343 (2019). PubMed: 31345079