Overview

  • Product name
    Anti-NeuN antibody - Neuronal Marker
    See all NeuN primary antibodies
  • Description
    Rabbit polyclonal to NeuN - Neuronal Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Cat, Dog, Common marmoset
    Predicted to work with: Horse, Cow, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse NeuN aa 300 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab155068)

  • Positive control
    • This antibody gave a positive signal in normal Rat Brain formalin fixed paraffin embedded tissue section within Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab128886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 25137049
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.

tissue cryoprotected in 25%Sucrose and OCT after brief fixation in 4%PFA (see Abreview)

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 34 kDa).

Target

Images

  • IHC-P image of FOX3/NeuN staining on mouse cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • All lanes : Anti-NeuN antibody - Neuronal Marker (ab128886) at 5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 48 kDa (possible isoform). We are unsure as to the identity of these extra bands.



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab128886 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • IHC-P image of FOX3/NeuN staining on rat brain sections using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.
  • IHC-P image of FOX3/NeuN staining on dog cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • Immunohistochemical analysis of mouse hippocampus, labeling NeuN with ab128886 (red). Tissue sections were blocked with 5% normal donkey seru, in 0.3% Triton/PBS for an hour. Immunostaining with anti-NeuN diluted 1/500 overnight at 4°C.

  • IHC-P image of FOX3/NeuN staining on cat cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • Immunohistochemical analysis of mouse cerebellar tumour frozen tissue section, labeling NeuN with ab128886. Samples were fixed in paraformaldehyde and blocked in 100% I-Block for 1 hour at room temperature. Incubation with ab128886 diluted 1/1000 for 24 hours at 4°C.

    See Abreview

  • IHC-P image of FOX3/NeuN staining on marmoset (common) cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • IHC-P image of FOX3/NeuN staining on goat cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • IHC-P image of FOX3/NeuN staining on rat brain sections of Cortex (upper image) and lateral ventricle - SVZ (lower image) using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • ab128886 staining FOX3/NeuN in Mouse granule neuron cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS and blocked with 10% serum for 60 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton + 3% normal Goat serum) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody. The Bergmann glia cells were stained with ab79351 against SOX2 shown in red. Nuclei were counterstained with DAPI.

    See Abreview

References

This product has been referenced in:
  • Nyamugenda E  et al. Injury to hypothalamic Sim1 neurons is a common feature of obesity by exposure to high-fat diet in male and female mice. J Neurochem 149:73-97 (2019). Read more (PubMed: 30615192) »
  • Kojima K  et al. Selective Ablation of Tumorigenic Cells Following Human Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cell Transplantation in Spinal Cord Injury. Stem Cells Transl Med 8:260-270 (2019). Read more (PubMed: 30485733) »
See all 11 Publications for this product

Customer reviews and Q&As

11-11 of 11 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Feb 18 2013

11-11 of 11 Abreviews or Q&A

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