Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted composite image of rat brain frontal cortex ( upper ) and lateral ventricle ( svz - lower) shows apropriate, specific positivity.
Note that progenitor neurones in the svz are negative, as expected.

Mr. Carl Hobbs

Verified customer

Submitted Feb 18 2013

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