Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid pH6
Permeabilization
No
Blocking step
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent (Ventana)

Secondary antibody

Name
Non-Abcam antibody was used: DAB-Polymer Detection Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
The image shows specifically labelled cerebellar granule neurons in an adult murine Cerebellum.
Interneurons within the molecular layer are negative.

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Submitted May 02 2013

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