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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid pH6
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent (Ventana)
Non-Abcam antibody was used: DAB-Polymer Detection Kit
Host species: Goat
Conjugation: Horse Radish Peroxidase
The image shows specifically labelled cerebellar granule neurons in an adult murine Cerebellum.
Interneurons within the molecular layer are negative.
Abcam user community
Submitted May 02 2013