Immunocytochemistry/ Immunofluorescence abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (granule neuron cells)
Specification
granule neuron cells
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS + 0.3% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.3% Triton + 3% NGS

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen goat anti rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
The image shows cerebellar granule neurons that are positive for NeuN (Alexa488). Bergmann glia cells are stained for Sox2 (Anti-SOX2 antibody [9-9-3] (ab79351) in red.
Nuclei are counterstained with DAPI.

Abcam user community

Verified customer

Submitted Apr 23 2013

Sign up