Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
None
Sample
Sheep Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - triton-x
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% normal serum in 0.1<
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: vectorlabs anti rabbit IgG biotinylated
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400

Additional data

Additional Notes
Works very well in free floating sections with no antigen retrieval. Also works on slide mounted sections after heat mediated antigen retrieval in pH 6.0 citrate buffer

Miss. Cathy Wyse

Verified customer

Submitted May 28 2014

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