Anti-NeuN antibody - Neuronal Marker (ab134014)

Chicken polyclonal NeuN antibody. Validated in WB, IHC, ICC and tested in Mouse, Human. Cited in 6 publication(s). Independently reviewed in 7 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-NeuN antibody - Neuronal Marker
    See all NeuN primary antibodies
  • Description

    Chicken polyclonal to NeuN - Neuronal Marker
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to NeuN (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Corresponding to a region within Human (NP_001076044) and Mouse (NP_001020102).

  • Positive control

    • This product gave a signal in Western Blot in the following tissue lysates: Mouse Brain

Properties

Applications

Our Abpromise guarantee covers the use of ab134014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2000.
IHC-Fr 1/1000 - 1/2000.
ICC 1/1000 - 1/2000.
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (Alexa Fluor® 594) (ab207279).

Target

  • Function

    RNA-binding protein that regulates alternative splicing events.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ56884 antibody
    • FLJ58356 antibody
    • Fox-1 homolog C antibody
    • fox1 homolog C antibody
    • Fox3 antibody
    • FOX3NeuN antibody
    • hexaribonucleotide binding protein 3 antibody
    • HRNBP3 antibody
    • NEUN antibody
    • neuronal nuclei antibody
    • Rbfox3 antibody
    • RFOX3_HUMAN antibody
    • RNA binding protein fox-1 homolog 3 antibody
    • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
    see all

Images

  • All lanes : Anti-NeuN antibody - Neuronal Marker (ab134014) at 1/1000 dilution

    Lane 1 : Mouse Brain
    Lane 2 : Rat Brain

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse cerebral cortex tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.

  • Immunohistochemistry (Frozen sections) analysis of e18 mouse cochlear ganglion tissue staining NeuN (green) with ab134014. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin 3.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse CA1 hippocampus tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.

References

This product has been referenced in:

  • Lin JJ  et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). Read more (PubMed: 28656058) »
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF . Read more (PubMed: 27592818) »
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain)
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 29 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain Tissue)
Permeabilization
Yes - Triton X-100
Specification
Brain Tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 06 2018

Application
IHC - Wholemount
Sample
Rat Tissue (Inferior Colliculus)
Specification
Inferior Colliculus

Lauren Robinson

Verified customer

Submitted Feb 27 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.5% TWEEN
Specification
Brain
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 26 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 08 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jul 28 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse, whole brain sections, striatum)
Antigen retrieval step
None
Permeabilization
Yes - Tween-20
Specification
Mouse, whole brain sections, striatum
Blocking step
Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2013

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