Product nameAnti-NeuN antibody - Neuronal Marker
See all NeuN primary antibodies
DescriptionChicken polyclonal to NeuN - Neuronal Marker
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to NeuN (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Corresponding to a region within Human (NP_001076044) and Mouse (NP_001020102).
- This product gave a signal in Western Blot in the following tissue lysates: Mouse Brain
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.9% Sodium chloride
Concentration information loading...
Purification notesab134014 was affinity-purified from the IgY fraction prepared from eggs liad by immunised hens. The final product is a mixture of affinity-purified NeuN antibodies (25 µg/ml), NeuN IgY fraction (10 mg/ml).
ELISA pair antibody
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab134014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2000.|
|IHC-Fr||1/1000 - 1/2000.|
|ICC||1/1000 - 1/2000.|
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (Alexa Fluor® 594) (ab207279).|
FunctionRNA-binding protein that regulates alternative splicing events.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
All lanes : Anti-NeuN antibody - Neuronal Marker (ab134014) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse cerebral cortex tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.
Immunohistochemistry (Frozen sections) analysis of e18 mouse cochlear ganglion tissue staining NeuN (green) with ab134014. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin 3.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse CA1 hippocampus tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.
This product has been referenced in:
- Lin JJ et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). Read more (PubMed: 28656058) »
- Shi W et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF . Read more (PubMed: 27592818) »