Key features and details
- Rabbit polyclonal to Neuraminidase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Neuraminidase antibody
See all Neuraminidase primary antibodies
DescriptionRabbit polyclonal to Neuraminidase
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Orangutan
Recombinant fragment corresponding to Human Neuraminidase aa 156-415.
SLCAHKAGCQVASTMLVWSKDDGVSWSTPRNLSLDIGTEVFAPGPGSGIQ KQREPRKGRLIVCGHGTLERDGVFCLLSDDHGASWRYGSGVSGIPYGQPK QENDFNPDECQPYELPDGSVVINARNQNNYHCHCRIVLRSYDACDTLRPR DVTFDPELVDPVVAAGAVVTSSGIVFFSNPAHPEFRVNLTLRWSFSNGTS WRKETVQLWPGPSGYSSLATLEGSMDGEEQAPQLYVLYEKGRNHYTESIS VAKISVYGTL
Database link: Q99519
- WB: Mouse liver tissue extract; rat liver tissue extract. IHC-P: Human adrenal tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab230526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 45 kDa.|
FunctionCatalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
Tissue specificityHighly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
Involvement in diseaseDefects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells.
Sequence similaritiesBelongs to the glycosyl hydrolase 33 family.
Contains 4 BNR repeats.
DomainA C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.
Cellular localizationLysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
- Information by UniProt
- Acetylneuraminyl hydrolase antibody
- exo-alpha-sialidase antibody
- G9 sialidase antibody
All lanes : Anti-Neuraminidase antibody (ab230526) at 1/500 dilution
Lane 1 : Mouse liver tissue extract
Lane 2 : Rat liver tissue extract
Developed using the ECL technique.
Predicted band size: 45 kDa
Formalin-fixed, paraffin-embedded human adrenal tissue stained for Neuraminidase with ab230526 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230526 has not yet been referenced specifically in any publications.