Anti-Neuraminidase antibody (ab244386)
Key features and details
- Rabbit polyclonal to Neuraminidase
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Neuraminidase antibody
See all Neuraminidase primary antibodies -
Description
Rabbit polyclonal to Neuraminidase -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Human -
Immunogen
Recombinant fragment corresponding to Human Neuraminidase aa 200-400.
Database link: Q99519 -
Positive control
- ICC/IF: U-251 MG cells. IHC-P: Human kidney, liver, and placenta tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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All applications |
Mouse
Rat
Cow
Pig
Orangutan
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Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage. -
Tissue specificity
Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain. -
Involvement in disease
Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells. -
Sequence similarities
Belongs to the glycosyl hydrolase 33 family.
Contains 4 BNR repeats. -
Domain
A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes. -
Post-translational
modificationsN-glycosylated.
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane. -
Cellular localization
Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. - Information by UniProt
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Database links
- Entrez Gene: 505554 Cow
- Entrez Gene: 4758 Human
- Entrez Gene: 18010 Mouse
- Entrez Gene: 100172668 Orangutan
- Entrez Gene: 100124381 Pig
- Entrez Gene: 24591 Rat
- Omim: 608272 Human
- SwissProt: A6BMK7 Cow
see all -
Alternative names
- Acetylneuraminyl hydrolase antibody
- exo-alpha-sialidase antibody
- G9 sialidase antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Neuraminidase (green) using ab244386 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuraminidase antibody (ab244386)
Paraffin-embedded human placenta tissue stained for Neuraminidase using ab244386 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuraminidase antibody (ab244386)Paraffin-embedded human liver tissue stained for Neuraminidase using ab244386 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuraminidase antibody (ab244386)Paraffin-embedded human kidney tissue stained for Neuraminidase using ab244386 at 1/20 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244386 has not yet been referenced specifically in any publications.