Recombinant
RabMAb

Recombinant Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)

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Overview

  • Product name

    Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free
    See all Neuraminidase primary antibodies

  • Description

    Rabbit monoclonal [EPR15712] to Neuraminidase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Neuraminidase aa 50-150. The exact sequence is proprietary.
    Database link: Q99519

  • General notes

    Ab251229 is the carrier-free version of ab197020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251229 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab251229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.

    • Tissue specificity

      Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.

    • Involvement in disease

      Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells.

    • Sequence similarities

      Belongs to the glycosyl hydrolase 33 family.
      Contains 4 BNR repeats.

    • Domain

      A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.

    • Post-translational
      modifications

      N-glycosylated.
      Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.

    • Cellular localization

      Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
    • Information by UniProt

    • Database links

      • Alternative names

        • Acetylneuraminyl hydrolase antibody
        • exo-alpha-sialidase antibody
        • G9 sialidase antibody
        • Lysosomal sialidase antibody
        • N acetyl alpha neuraminidase 1 antibody
        • N-acetyl-alpha-neuraminidase 1 antibody
        • NANH antibody
        • NEU antibody
        • NEU1 antibody
        • NEUR1_HUMAN antibody
        • Neuraminidase 1 antibody
        • Neuraminidase antibody
        • SIAL1 antibody
        • sialidase 1 (lysosomal sialidase) antibody
        • Sialidase 1 antibody
        • Sialidase, lysosomal antibody
        • Sialidase-1 antibody
        see all

      References

      ab251229 has not yet been referenced specifically in any publications.

      Publishing research using ab251229? Please let us know so that we can cite the reference in this datasheet.

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