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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)

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Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
  • Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
  • Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15712] to Neuraminidase - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human Neuraminidase protein (ab131685)

View more associated products

Overview

  • Product name

    Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free
    See all Neuraminidase primary antibodies
  • Description

    Rabbit monoclonal [EPR15712] to Neuraminidase - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251229 is the carrier-free version of ab197020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251229 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15712
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-Neuraminidase antibody [EPR15712] (ab197020)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human Neuraminidase protein (ab131685)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
    • Tissue specificity

      Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
    • Involvement in disease

      Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells.
    • Sequence similarities

      Belongs to the glycosyl hydrolase 33 family.
      Contains 4 BNR repeats.
    • Domain

      A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.
    • Post-translational
      modifications

      N-glycosylated.
      Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.
    • Cellular localization

      Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
    • Target information above from: UniProt accession Q99519 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4758 Human
      • Omim: 608272 Human
      • SwissProt: Q99519 Human
      • Unigene: 520037 Human
      • Alternative names

        • Acetylneuraminyl hydrolase antibody
        • exo-alpha-sialidase antibody
        • G9 sialidase antibody
        • Lysosomal sialidase antibody
        • N acetyl alpha neuraminidase 1 antibody
        • N-acetyl-alpha-neuraminidase 1 antibody
        • NANH antibody
        • NEU antibody
        • NEU1 antibody
        • NEUR1_HUMAN antibody
        • Neuraminidase 1 antibody
        • Neuraminidase antibody
        • SIAL1 antibody
        • sialidase 1 (lysosomal sialidase) antibody
        • Sialidase 1 antibody
        • Sialidase, lysosomal antibody
        • Sialidase-1 antibody
        see all

      Images

      • Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
        Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
        All lanes : Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/2000 dilution

        Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
        Lane 2 : Human fetal liver lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 45 kDa
        Observed band size: 45 kDa


        Exposure time: 3 minutes


        This data was developed using ab197020, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
        Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
        Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 45 kDa
        Observed band size: 45 kDa


        Exposure time: 3 minutes


        This data was developed using ab197020, the same antibody clone in a different buffer formulation.

         

      • Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
        Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (0)

      Publishing research using ab251229? Please let us know so that we can cite the reference in this datasheet.

      ab251229 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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