Recombinant Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
![Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)](https://www.abcam.com/ps/products/251/ab251229/Images/ab251229-403465-anti-neuraminidase-antibody-epr15712-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15712] to Neuraminidase - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free
See all Neuraminidase primary antibodies -
Description
Rabbit monoclonal [EPR15712] to Neuraminidase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251229 is the carrier-free version of ab197020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab251229 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15712 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage. -
Tissue specificity
Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain. -
Involvement in disease
Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells. -
Sequence similarities
Belongs to the glycosyl hydrolase 33 family.
Contains 4 BNR repeats. -
Domain
A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes. -
Post-translational
modificationsN-glycosylated.
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane. -
Cellular localization
Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. - Information by UniProt
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Database links
- Entrez Gene: 4758 Human
- Omim: 608272 Human
- SwissProt: Q99519 Human
- Unigene: 520037 Human
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Alternative names
- Acetylneuraminyl hydrolase antibody
- exo-alpha-sialidase antibody
- G9 sialidase antibody
see all
Images
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Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)All lanes : Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutesThis data was developed using ab197020, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)](https://www.abcam.com/ps/products/251/ab251229/Images/ab251229-399055-anti-neuraminidase-antibody-epr15712-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutesThis data was developed using ab197020, the same antibody clone in a different buffer formulation.
-
![Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)](https://www.abcam.com/ps/products/251/ab251229/Images/ab251229-3-benefits-of-recombinant-antibodies.png)
Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (ab251229)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab251229 has not yet been referenced specifically in any publications.