Key features and details
- Rabbit polyclonal to Neurexin 1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Neurexin 1 antibody
See all Neurexin 1 primary antibodies
DescriptionRabbit polyclonal to Neurexin 1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Neurexin 1 aa 1219-1398.
RSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQGQPFQGQLS GLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSSMTTESTATAMQSEMS TSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPC EPSSGGLANPTRAGGREPYPGSAEVIRESS
Database link: Q9ULB1
- ICC/IF: HepG2 cells. WB: Mouse heart and kidney lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/2000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
FunctionCell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom.
Sequence similaritiesBelongs to the neurexin family.
Contains 3 EGF-like domains.
Contains 6 laminin G-like domains.
Cellular localizationCell membrane. Cell junction, synapse. Localized on the pre-synaptic membrane.
- Information by UniProt
FormThe neurexin genes use alternate promoters, splice sites and exons to encode potentially hundreds or thousands of mRNA transcripts.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Neurexin I beta (green) using ab222806 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
All lanes : Anti-Neurexin 1 antibody (ab222806) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
ab222806 has not yet been referenced specifically in any publications.