Overview

  • Product name

  • Description

    Goat polyclonal to Neurexin 1
  • Host species

    Goat
  • Specificity

    This antibody will detect Neurexin 1 beta and Neurexin 1 alpha (isoform 1a, 2a and 3a).
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Neurexin I beta (C terminal). The immunogen sequence is aa419-432 of Neurexin 1 beta (P58400), aa1454-1467 of Neurexin 1 alpha (Q9ULB1), aa1476-1489 of Neurexin 1 alpha (Q9ULB1-2), and aa1524-1537 of Neurexin 1 alpha (Q9ULB1-3).
    Sequence:

    KEKQPSSAKSSNKN

  • Positive control

    • Human brain (cerebellum) lysate IF/ICC: SKNSH cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab77596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000
    WB: Use at a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Cell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom.
    • Tissue specificity

      Brain.
    • Sequence similarities

      Belongs to the neurexin family.
      Contains 3 EGF-like domains.
      Contains 6 laminin G-like domains.
    • Post-translational
      modifications

      N-glycosylated.
      O-glycosylated.
    • Cellular localization

      Cell membrane. Cell junction, synapse. Localized on the pre-synaptic membrane.
    • Information by UniProt
    • Database links

    • Form

      The neurexin genes use alternate promoters, splice sites and exons to encode potentially hundreds or thousands of mRNA transcripts.
    • Alternative names

      • DKFZp313P2036 antibody
      • FLJ35941 antibody
      • Hs.22998 antibody
      • KIAA0578 antibody
      • Neurexin 1 alpha antibody
      • Neurexin 1 beta antibody
      • Neurexin I-alpha antibody
      • Neurexin-1 antibody
      • Neurexin-1-alpha antibody
      • NRX1A_HUMAN antibody
      • NRXN1 antibody
      • PTHSL2 antibody
      • SCZD17 antibody
      see all

    Images

    • ab77596 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA  / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77596 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 

      Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-Neurexin 1 antibody (ab77596) at 0.05 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 162 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?



      Primary antibody incubation: 1 hour

    References

    This product has been referenced in:

    • Torres-Vega E  et al. Netrin-1 receptor antibodies in thymoma-associated neuromyotonia with myasthenia gravis. Neurology 88:1235-1242 (2017). Read more (PubMed: 28251919) »
    • Wei H  et al. Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells. Sci Rep 6:33402 (2016). WB . Read more (PubMed: 27624276) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Beta cell)
    Permeabilization
    Yes - 0.1% saponin
    Specification
    Beta cell
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 24 2018

    Answer



    There seems to be a few different isoforms of Neurexin I beta. For example, P58400 (NRX1B_HUMAN) notes a protein of nearly 47kDa. Another form, Q9ULB1 (NRX1A_HUMAN), is listed as nearly 162kDa, which is the form referred to on the online product datasheet. Here is a link to the Uniprot entry: http://www.uniprot.org/uniprot/Q9ULB1#section_seq.

    Read More

    Answer

    Thank you for your message. I am very pleased to hear the customer would like to make use of our testing discount offer and test ab77596 in ICC/IF.

    DISCOUNT CODE: ####
    Expiration date: ####

    This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC-IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

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