Product nameAnti-Neurexin 1 antibody
See all Neurexin 1 primary antibodies
DescriptionGoat polyclonal to Neurexin 1
SpecificityThis antibody will detect Neurexin 1 beta and Neurexin 1 alpha (isoform 1a, 2a and 3a).
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
Synthetic peptide corresponding to Human Neurexin I beta (C terminal). The immunogen sequence is aa419-432 of Neurexin 1 beta (P58400), aa1454-1467 of Neurexin 1 alpha (Q9ULB1), aa1476-1489 of Neurexin 1 alpha (Q9ULB1-2), and aa1524-1537 of Neurexin 1 alpha (Q9ULB1-3).
- Human brain (cerebellum) lysate IF/ICC: SKNSH cell line.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom.
Sequence similaritiesBelongs to the neurexin family.
Contains 3 EGF-like domains.
Contains 6 laminin G-like domains.
Cellular localizationCell membrane. Cell junction, synapse. Localized on the pre-synaptic membrane.
- Information by UniProt
FormThe neurexin genes use alternate promoters, splice sites and exons to encode potentially hundreds or thousands of mRNA transcripts.
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ab77596 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77596 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-Neurexin 1 antibody (ab77596) at 0.05 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary antibody incubation: 1 hour
This product has been referenced in:
- Torres-Vega E et al. Netrin-1 receptor antibodies in thymoma-associated neuromyotonia with myasthenia gravis. Neurology 88:1235-1242 (2017). Read more (PubMed: 28251919) »
- Wei H et al. Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells. Sci Rep 6:33402 (2016). WB . Read more (PubMed: 27624276) »