Product nameAnti-Neurexin II alpha antibody
DescriptionRabbit polyclonal to Neurexin II alpha
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- normal adult human cerebral cortex SKNSH cell line
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab34245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/700. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
RelevanceNeurexin 2 alpha belongs to the Neurexin family, a group of neuronal proteins that function as cell adhesion molecules during synaptogenesis and intracellular signaling. Mammalian genomes contain just three neurexin genes. However, many (>200) neurexin isoforms may be expressed through the use of two alternative promoters and alternative splicing at up to five different positions.
Cellular localizationMembrane; single-pass type I membrane protein.
- Neurexin 2 antibody
- Neurexin II antibody
- NRXN2 antibody
(Image courtesy of Human Protein Atlas).
ab34245 staining Neurexin II alpha protein in normal adult human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab34245 (1/700 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Anti-Neurexin II alpha antibody (ab34245) at 1/400 dilution + whole cell lysate prepared from murine N2A cells at 20 µg
Goat polyclonal conjugated to Dylight 800 at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Samples were blocked using 0.2% casein for 1 hour at 37°C.
ICC/IF image of ab34245 stained SKNSH cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34245, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Harkin LF et al. Neurexins 1-3 Each Have a Distinct Pattern of Expression in the Early Developing Human Cerebral Cortex. Cereb Cortex 27:216-232 (2017). Read more (PubMed: 28013231) »
- Brito-Moreira J et al. Interaction of amyloid-ß (Aß) oligomers with neurexin 2a and neuroligin 1 mediates synapse damage and memory loss in mice. J Biol Chem 292:7327-7337 (2017). Read more (PubMed: 28283575) »