Anti-NEURL1B antibody (ab122400)
Key features and details
- Rabbit polyclonal to NEURL1B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEURL1B antibody -
Description
Rabbit polyclonal to NEURL1B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: ALIDVYGITD EVQLLESAFA DTLTPARLSQ ARFSACLPPS SHDAANFDNN ELENNQVVAK LGHL, corresponding to amino acids 178-241 of Human NEURL1B according to A8MQ27
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Positive control
- Human rectum tissue; Human liver, Human tonsil, RT-4 and U-251 MG cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 2 NHR (neuralized homology repeat) domains.
Contains 1 RING-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 54492 Human
- SwissProt: A8MQ27 Human
- Unigene: 91521 Human
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Alternative names
- E3 ubiquitin-protein ligase NEURL1B antibody
- E3 ubiquitin-protein ligase NEURL3 antibody
- hNeur2 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251 MG shows positivity in vesicles. Recommended concentration of ab122400 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122400, at a 1/225 dilution, staining NEURL1B in glandular cells of paraffin-embedded Human rectum tissue by immunohistochemistry.
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All lanes : Anti-NEURL1B antibody (ab122400) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122400 has not yet been referenced specifically in any publications.