Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 43 kDa.Can be blocked with Human Neuro D4 peptide (ab22971).|
FunctionMay have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Sequence similaritiesBelongs to the requiem/DPF family.
Contains 2 PHD-type zinc fingers.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- BAF45B antibody
- BRG1-associated factor 45B antibody
- D4 antibody
Anti-Neuro D4 antibody (ab3940) at 1 µg/ml + Human Brain (Cerebellum) lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab3940 has not yet been referenced specifically in any publications.