Overview

  • Product name

    Anti-Neurobeachin antibody
    See all Neurobeachin primary antibodies
  • Description

    Goat polyclonal to Neurobeachin
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise isoform 1 (NP_056493.3) and isoform 2 (NP_001191126.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pufferfish, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-DFNRWHYEHQNRY

    , corresponding to C terminal amino acids 2934-2946 of Human Neurobeachin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    This antibody gave a positive result in Peptide ELISA against the immunizing peptide. Antibody detection limit dilution 1/8000.


    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 327 kDa). Approx 300 kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins.
    • Tissue specificity

      Predominant in many brain structures. Also expressed at medium levels in spleen, thymus, prostate, testis and ovary. Low level expression is seen in heart, kidney, pancreas, skeletal muscle and intestine.
    • Sequence similarities

      Belongs to the WD repeat neurobeachin family.
      Contains 1 BEACH domain.
      Contains 5 WD repeats.
    • Domain

      RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    • Cellular localization

      Cytoplasm. Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BCL8B antibody
      • FLJ10197 antibody
      • KIAA1544 antibody
      • Lysosomal trafficking regulator 2 antibody
      • Lysosomal-trafficking regulator 2 antibody
      • LYST2 antibody
      • NBEA antibody
      • NBEA_HUMAN antibody
      • Neurobeachin antibody
      • Protein BCL8B antibody
      • Protein neurobeachin antibody
      • RP11-270C18.1 antibody
      see all

    Images

    • ab58563 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    • All lanes : Anti-Neurobeachin antibody (ab58563) at 1 µg/ml

      Lane 1 : Lysate prepared from non transfected HEK293 T cells
      Lane 2 : Lysate prepared from HEK293 T cells transfected with mouse Neurobeachin

      Developed using the ECL technique.

      Predicted band size: 327 kDa
      Observed band size: 300 kDa
      why is the actual band size different from the predicted?

    References

    ab58563 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab58563.
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