Overview

  • Product name

    Anti-Neurocan antibody [650.24]
    See all Neurocan primary antibodies
  • Description

    Mouse monoclonal [650.24] to Neurocan
  • Host species

    Mouse
  • Specificity

    Specific for Neurocan core protein; also reacts with neurocan C fragment
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Embryonic rat brain proteoglycans

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    650.24
  • Myeloma

    P3x63
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 143 kDa.

Target

  • Function

    May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

Images

  • ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.

    See Abreview

References

This product has been referenced in:

  • Li G  et al. Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. Front Cell Neurosci 10:241 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27807407) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

According to the Unigen expression profile of this protein, a good positive control for this protein could be brain or vascular mouse tissue lysates.

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.169047

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 23 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioblastoma cell line C6)
Specification
glioblastoma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 23 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioma cell line C6)
Specification
glioma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5%

Abcam user community

Verified customer

Submitted Aug 31 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line D54MG)
Specification
human glioblastoma cell line D54MG
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 31 2011

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