Anti-Neurocan antibody [650.24] (ab31979)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Neurocan antibody [650.24]
See all Neurocan primary antibodies -
Description
Mouse monoclonal [650.24] to Neurocan -
Host species
Mouse -
Specificity
Specific for Neurocan core protein; also reacts with neurocan C fragment -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Embryonic rat brain proteoglycans
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
650.24 -
Myeloma
P3x63 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 143 kDa. |
Target
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Function
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1463 Human
- Entrez Gene: 13004 Mouse
- Entrez Gene: 58982 Rat
- Omim: 600826 Human
- SwissProt: O14594 Human
- SwissProt: P55066 Mouse
- SwissProt: P55067 Rat
- Unigene: 169047 Human
see all -
Alternative names
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (ab31979)](https://www.abcam.com/ps/products/31/ab31979/Images/ab31979-43568-anti-neurocan-antibody-65024-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (ab31979)Image courtesy of an anonymous Abreview.ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.
References
This product has been referenced in:
- Li G et al. Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. Front Cell Neurosci 10:241 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27807407) »
