Recombinant Anti-Neurocan antibody [EPR6397] (ab125021)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6397] to Neurocan
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Neurocan antibody [EPR6397]
See all Neurocan primary antibodies -
Description
Rabbit monoclonal [EPR6397] to Neurocan -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Neurocan aa 1-100. The exact sequence is proprietary.
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Positive control
- Human hippocampus and hypothalamus lysates; Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6397 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1463 Human
- Omim: 600826 Human
- SwissProt: O14594 Human
- Unigene: 169047 Human
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Alternative names
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
see all
Images
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All lanes : Anti-Neurocan antibody [EPR6397] (ab125021) at 1/10000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 130,270 kDa why is the actual band size different from the predicted? -
ab125021, at 1/250 dilution, staining Neurocan in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab125021 has been referenced in 2 publications.
- Su H et al. TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation via the Smurf2/ALK5 Axis. Front Cell Neurosci 15:569424 (2021). PubMed: 33815059
- Silver DJ et al. Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment. J Neurosci 33:15603-17 (2013). Human . PubMed: 24068827